Objective. Students will select and apply appropriate powder, cyanoacrylate fuming, and small particle reagent techniques to non-porous items and document results with photography and comparison notes.
This lesson, its worked examples and its practice questions has not yet been verified by a licensed professional in the field or by a certification or approval body. It is a study draft and may contain errors. Do not use it as instructions for work on a job site or on live equipment. Always follow your instructor, your supervising professional, and current official references and regulations.
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Latent fingerprints on non-porous surfaces are among the most common and recoverable types of trace evidence at crime scenes. Non-porous surfaces — glass, plastic, metal, and coated card — do not absorb sweat and sebaceous residue, so the deposit sits on top of the surface rather than soaking in. That physical fact drives every technique choice you will make in this lab.
Before you touch a brush or open a fuming chamber, you must assess four variables: surface color, surface texture, contamination level, and whether the item can safely enter a fuming chamber. A decision matrix forces you to think through all four before you commit. Choosing the wrong technique can obscure or destroy the print permanently — you only get one chance.
CA fuming is the cornerstone technique for non-porous items and is tested on the IAI CCSI exam. The process must be conducted inside a dedicated fuming chamber — never in open air.
Safety first: Cyanoacrylate vapor is an irritant to eyes and respiratory mucous membranes. Before opening any container or operating the chamber:
1. Confirm the chamber exhaust fan is functional and vented to the outside or through an activated-carbon filter.
2. Wear chemical splash goggles (not safety glasses), nitrile gloves, and a lab coat or disposable coverall.
3. Never lean over an open fuming chamber while it is active.
4. Know the location of the eyewash station before you begin.
Procedure:
1. Suspend or position items inside the chamber so all surfaces are exposed to vapor.
2. Place the cyanoacrylate adhesive on the heating element or use a pre-measured one-use packet; do not overfill.
3. Place a small cup of warm water in the chamber to raise humidity — this accelerates polymerization.
4. Seal the chamber and run it according to your laboratory's SOP (typically until white ridge detail is visible, or for a set time cycle).
5. Remove items carefully; handle with gloves to prevent adding new prints.
After fuming, items with complex or dark backgrounds benefit from fluorescent dye staining. Rhodamine 6G (or an equivalent such as Ardrox or Basic Yellow 40) in solution is applied by spraying, dipping, or brushing, allowed to dwell briefly, and then rinsed with clean water or methanol depending on the dye used. The dye binds to the CA polymer. Under an ALS (typically in the 490–550 nm range for Rhodamine 6G, viewed through an orange barrier filter), the ridges fluoresce brightly against a dark background.
PPE note: Fluorescent dye solutions may be flammable if solvent-based. Check the SDS before use. Wear chemical goggles and gloves; use the ALS only with the manufacturer-specified barrier goggles — never look at the ALS beam directly.
Documentation is as important as development. A print that is not photographed properly is legally and scientifically useless. Follow this sequence:
1. Photograph the item in context (overall and mid-range) before disturbing it.
2. Attach a scale (ruler or ABFO No. 2 scale) adjacent to — never overlapping — the print.
3. Use a DSLR with a macro lens; set the camera perpendicular to the print surface to avoid keystoning distortion.
4. Under white light: capture the print showing ridge detail.
5. Under ALS (if dye-stained or fluorescent powder was used): re-photograph; darken the room as much as possible.
6. Record exposure settings, light source, filter, and distance in your notes.
ACE (Analysis, Comparison, Evaluation) is the standard friction ridge examination methodology recognized by the Scientific Working Group for Friction Ridge Analysis, Study and Technology (SWGFAST) and required by the IAI.
Using precise ACE language in your written report is mandatory for the IAI Latent Print Certification and for courtroom testimony.
Answer each question, then check it. Nothing is recorded; this is your own practice.
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