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newSchoolWorks.com › VoTechWorks › Agriculture, Environment & Public Service › Crime Scene / Forensic Technician › Latent Prints: Development, Recovery, and Comparison › Lesson 8
AGR-CSI53-04 · Lesson 8 · 12 clock hours · demo

Alternate Light Source (ALS) and Fingerprint Photography

Objective. Students will configure an ALS unit at the correct wavelength and select the matching barrier filter to maximize print contrast, then produce a complete photographic series of a latent print that meets court-admissible documentation standards.

⚠ Pending professional review

This lesson, its worked examples and its practice questions has not yet been verified by a licensed professional in the field or by a certification or approval body. It is a study draft and may contain errors. Do not use it as instructions for work on a job site or on live equipment. Always follow your instructor, your supervising professional, and current official references and regulations.

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Alternate Light Source (ALS) and Fingerprint Photography

Why ALS Works

Latent fingerprints are invisible or nearly invisible under ordinary white light. An Alternate Light Source (ALS) exploits the fact that certain substances fluoresce — they absorb energy at one wavelength and re-emit it at a longer, lower-energy wavelength. When a fingerprint has been treated with a fluorescent developer such as cyanoacrylate (CA) followed by Rhodamine 6G, DFO, or a fluorescent powder, the developed ridge detail glows when struck by the correct excitation wavelength. The ALS provides that narrow-band excitation; a barrier filter placed over the camera lens (or worn as goggles) blocks the excitation light and passes only the fluorescent emission, making ridges appear bright against a dark background.

This principle sits squarely within the electromagnetic spectrum. Visible light runs roughly from 400 nm (violet) to 700 nm (red). ALS units produce selectable outputs across that range and into the near-ultraviolet and near-infrared. The key rule: the barrier filter must have a cut-on wavelength above the ALS output wavelength — it blocks excitation light but transmits the longer-wavelength fluorescent emission.

Matching Wavelength to Developer

Different fluorescent developers have different excitation and emission peaks. While your agency's documented protocols are authoritative, the general relationships are well established:

Always consult the SDS and your agency's validated protocol before use. Never assume one wavelength fits all developers.

Safety First

ALS units emit concentrated light that can cause serious eye injury, particularly at short ultraviolet and blue-violet wavelengths.

Camera Setup for ALS Photography

ALS photography in a darkened environment requires deliberate camera settings. Auto-exposure will almost always fail.

ISO: Use the lowest native ISO your DSLR offers (commonly ISO 100 or 200). A high ISO increases noise and obscures fine ridge detail.

Aperture: A mid-range aperture (f/8 to f/16 on a macro lens) maximizes depth of field so that ridge detail across the print surface is sharp.

Shutter speed: Because the room is dark and exposures may be long (several seconds), you must use a tripod and a cable or remote shutter release to eliminate camera shake. Trial exposures are normal; bracket your exposures.

Focus: Use manual focus. Autofocus systems struggle in low light and can hunt or lock onto the wrong plane. Use live view magnified to the ridge level to confirm critical focus before capturing.

White balance: Set a custom white balance or use a fixed preset appropriate to the ALS wavelength output. Auto white balance can shift color unpredictably and reduce ridge contrast.

Barrier filter on lens: Attach or hold the barrier filter over the lens for all ALS exposures. Remove it for white-light comparison shots.

The Court-Admissible Documentation Series

Forensic photography follows a systematic sequence to establish context and ensure the image can be admitted as evidence. For a latent print, the required series is:

1. Overall/Orientation shot — shows the case label, item, and surrounding scene. The case identifier must be legible.

2. Mid-range shot — shows the print's location on the item.

3. Close-up under white light, without scale — captures ridge detail without obstruction.

4. Close-up under white light, with ABFO No. 2 scale — the scale provides size reference and must be in the same plane as the print.

5. Close-up under ALS, without scale — fluorescent ridge detail.

6. Close-up under ALS, with ABFO No. 2 scale — fluorescent detail with size reference.

Never move the scale between shots 4 and 6 if the print has not moved. Record all camera settings, ALS wavelength, and filter used in your case notes. Images are saved to a write-protected or hashed media card as part of chain-of-custody practice.

Connecting to Your Credentials

The IAI CCSI and Latent Print certifications both evaluate your ability to properly document and recover latent prints using validated methods. Employers and courts expect technicians to understand why each step is taken, not just follow a checklist. A well-executed ALS photographic series is one of the most common deliverables you will produce in this trade.

Key terms8

Alternate Light Source (ALS)A device that produces selectable narrow-band wavelengths of light used to excite fluorescent materials in latent fingerprint developers, making ridge detail visible against a dark background.
FluorescenceThe property of a substance to absorb light at one wavelength (excitation) and re-emit it at a longer, lower-energy wavelength (emission), which is the basis for ALS-assisted fingerprint visualization.
Barrier filterAn optical filter placed over the camera lens or worn as goggles that blocks the ALS excitation wavelength and passes only the longer-wavelength fluorescent emission, improving contrast.
Excitation wavelengthThe specific wavelength of light produced by the ALS that causes a fluorescent developer on the print to emit visible light; must be matched to the developer in use.
DFO (1,8-Diazafluoren-9-one)A fluorescent ninhydrin-analog chemical developer that reacts with amino acids in latent print residue and fluoresces under blue-violet ALS excitation.
ABFO No. 2 scaleA standardized photographic scale card with L-shaped reference markers, used in forensic photography to provide accurate size reference and check for lens distortion.
Physical developer (PD)A silver-based aqueous fingerprint development technique that deposits metallic silver on ridge residue; PD-developed prints do not fluoresce and are imaged under white raking light, not ALS.
Cable/remote shutter releaseA device that triggers the camera shutter without the photographer touching the camera body, eliminating vibration during long-exposure ALS photography.
In class: ALS theory and demonstration: instructor explains the electromagnetic spectrum in context of latent-print fluorescence, demonstrates wavelength selection for CA-stained prints (e.g., blue-green excitation) versus other fluorescent developers, and walks through the camera settings (low ISO, manual focus, tripod use) needed for dark-room ALS photography. · ALS configuration exercise: students are given a set of latent prints developed with four different fluorescent techniques and must independently select the correct ALS wavelength and barrier filter for each, justify their choice on a worksheet, then capture images and compare to instructor reference images. · Full documentation series: each student photographs one prepared print card using the full admissible-documentation sequence—overall scene orientation shot with case label, mid-range shot showing print location, close-up with and without scale under white light, close-up with and without scale under ALS—and submits a labeled image folder.
Materials: ALS unit with multiple wavelength outputs, barrier filter goggle set, DSLR camera, tripod, cable shutter release, ABFO No. 2 scale cards, darkened room or blackout curtain, prints developed with CA + Rhodamine, DFO, fluorescent powder, and physical developer, worksheet for wavelength selection
Check: Wavelength/filter selection worksheet 80% correct; full documentation series reviewed against a 10-point checklist (orientation shot, mid-range shot, close-up with scale, close-up without scale, ALS images with and without scale, case label visible, sharp focus, correct white balance, no blown highlights, adequate ridge contrast)—8 of 10 criteria met to pass.

Practice check8

Answer each question, then check it. Nothing is recorded; this is your own practice.

Latent print development and recovery
1. A latent print has been developed with cyanoacrylate fuming followed by Rhodamine 6G staining. Which ALS wavelength range and barrier filter pairing is most appropriate for exciting this print?
Forensic photography and documentation
2. A student is photographing a fluorescent print under ALS in a darkened room and finds that every image is blurry even though the tripod is secured. What is the most likely cause?
Latent print development and recovery
3. Which of the following latent print developers does NOT produce fluorescence and therefore requires white oblique light rather than ALS for photography?
Forensic photography and documentation
4. What is the primary reason a forensic photographer uses the lowest available native ISO when capturing ALS images of a latent print?
Crime scene documentation and evidence collection
5. During the court-admissible documentation series, a technician skips the overall orientation shot and begins with a close-up. What is the principal evidentiary problem with this approach?
Forensic photography and documentation
6. A technician positions an ABFO No. 2 scale card next to a latent print for a close-up photograph but places it on a surface slightly higher than the print. What photographic error does this introduce?
Safety and personal protective equipment
7. Before activating an ALS unit set to a blue-violet wavelength, what is the most critical immediate safety action a technician must take?
Forensic photography and documentation
8. A student correctly captures five of six required images in the admissible-documentation series but forgets the mid-range shot. Using the 10-point checklist (8 of 10 required to pass), how does this single omission affect the student's result, assuming all other criteria are met?
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