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newSchoolWorks.com › VoTechWorks › Agriculture, Environment & Public Service › Crime Scene / Forensic Technician › Evidence Collection & Chain of Custody › Lesson 7
AGR-CSI53-03 · Lesson 7 · 15 clock hours · lab

Collection and Packaging of Trace, Impression, and Toolmark Evidence

Objective. Students will recover trace evidence using tape lifts, electrostatic lifting devices (ESDA), and vacuum collection; cast a two-dimensional impression in dental stone; and package toolmark evidence without altering the mark.

⚠ Pending professional review

This lesson, its worked examples and its practice questions has not yet been verified by a licensed professional in the field or by a certification or approval body. It is a study draft and may contain errors. Do not use it as instructions for work on a job site or on live equipment. Always follow your instructor, your supervising professional, and current official references and regulations.

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Collection and Packaging of Trace, Impression, and Toolmark Evidence

Trace, impression, and toolmark evidence can place a suspect at a scene, identify a weapon, or reconstruct a sequence of events. Because this evidence is often fragile and irreplaceable, every decision you make during collection and packaging directly affects whether it will be admissible and scientifically useful in court.

Trace Evidence: Tape Lifts and Vacuum Collection

Trace evidence includes fibers, hair, glass fragments, soil particles, and pollen. The two primary field-collection methods are tape lifts and vacuum collection.

Tape lifts use clear adhesive tape pressed flat against a surface and then mounted on a backing (typically acetate or a clear rigid sheet). Press with even, moderate pressure — do not rub or slide the tape. Lift in one smooth pull, then apply immediately to the backing to prevent curling and cross-contamination. Label the backing with case number, date, location, and the direction of the lift.

Vacuum collection uses a portable forensic vacuum fitted with a filter trap. Change the filter trap between each area sampled to prevent cross-contamination. Seal the used trap in a paper or glassine envelope immediately after collection. Never use plastic bags for organic trace evidence because moisture causes mold and degradation.

Electrostatic Lifting Device (ESDA) for Dust Impressions

An electrostatic lifting device (ESDA) creates a static charge that transfers a dusty or dry-residue footwear impression from a floor or hard surface onto a black lifting film. The process is non-destructive to the underlying surface.

Procedure:

1. Photograph the impression in place with a scale and without a scale before any lifting attempt. Use oblique (side) lighting to enhance detail.

2. Lay the lifting film over the impression without touching the film to the impression surface prematurely.

3. Apply the ESDA unit and activate the charge; the dust particles migrate to the film.

4. Photograph the film immediately after lifting — do not wait.

5. Place the film in a rigid, flat evidence container (such as a document box or rigid plastic sleeve) to prevent the film from flexing, which would crack and displace the dust pattern.

6. Complete the evidence form before the container is sealed.

Safety note: ESDA units carry a high-voltage electrostatic charge. Keep the unit away from flammable dusts, solvents, and oxygen-enriched environments. Follow the manufacturer's operating instructions. Do not use the device near electronic equipment that could be damaged by static discharge.

Casting Impression Evidence with Dental Stone

Dental stone (Type III or IV gypsum) is the standard casting material for two-dimensional and three-dimensional footwear and tire-track impressions in soil or sand. It produces a hard, dimensionally stable cast that preserves fine detail.

Mixing: Add powder to water — not water to powder — to reduce air bubbles. A typical working ratio is approximately 300–350 grams of powder to 100 mL of water, but always follow the manufacturer's instructions on the package, because ratios vary by product. Mix until the consistency resembles thick pancake batter with no lumps.

Pouring:

1. Do not pour directly onto the impression. Direct the stream against a spatula held just above the impression so the stone flows gently over the surface.

2. Pour from one end to avoid trapping air pockets.

3. After the impression is covered, add a reinforcing layer and embed a label strip in the wet stone.

4. Allow to cure undisturbed for a minimum of 30–45 minutes (longer in cold or wet conditions).

5. Excavate carefully from the perimeter inward. Do not pry from the center.

6. Brush off loose soil gently; do not wash the cast in the field, as washing can erode fine detail.

7. Allow the cast to continue curing for at least 24 hours before further cleaning.

Packaging: Wrap the cast in clean paper (not plastic) and place in a rigid cardboard box with foam padding on all sides. Label the box on all four sides.

Safety note: Dental stone powder is a respiratory irritant. Wear an N95 or higher respirator and nitrile gloves when mixing. Work upwind or in ventilated areas when outdoors.

Toolmark Evidence: Examination, Photography, and Packaging

A toolmark is any impression, cut, gouge, or abrasion left when a tool contacts a softer surface. Striations and class characteristics in a toolmark can be compared to a suspect tool by a toolmark examiner.

The cardinal rule: never place the suspect tool into the mark to test fit. This destroys both the evidence and the comparison value.

Photography: Place a photo scale parallel to the mark, fill the camera frame, and shoot perpendicular to the surface. Use oblique lighting to reveal depth and striations. Take several exposures at different lighting angles. Record focal length, lighting angle, and aperture in your notes.

Packaging a metal coupon:

1. Cut the coupon section containing the mark using a saw or shears applied well away from the mark zone — never cut through or across the mark itself.

2. Handle the coupon by its edges, using gloves, to preserve any latent prints or trace on the mark surface.

3. Do not touch, swab, or apply any reagent to the mark surface.

4. Wrap the marked surface in clean foam or acid-free paper so nothing contacts the mark.

5. Place in a rigid evidence box. Complete the chain-of-custody form before sealing.

Chain of Custody and Documentation

Every item collected must have an unbroken chain of custody: a continuous, documented record of who collected it, who handled it, where it was stored, and who received it next. Any gap in this chain can result in evidence being excluded at trial. Complete evidence forms in ink, never pencil. Errors are crossed out with a single line, initialed, and dated — never erased or whited out.

Key terms8

Trace evidenceMinute physical material transferred between persons, objects, or environments — such as fibers, hair, or soil — collected by tape lift, vacuum, or other non-destructive means.
Electrostatic lifting device (ESDA)An instrument that applies a high-voltage static charge to lift dust or dry-residue impressions from a surface onto a black lifting film without physically contacting the impression.
Dental stoneA high-strength gypsum casting material (Type III or IV) used to produce hard, dimensionally stable casts of footwear and tire-track impressions in soil or sand.
ToolmarkAny impression, cut, gouge, or abrasion created when a tool contacts a softer surface; class and individual characteristics in the mark can be compared to a suspect tool.
Oblique lightingA photography technique in which the light source is angled low and to the side of a surface to reveal texture, depth, and fine detail in impressions or marks.
Chain of custodyThe continuous, documented record of every person who collected, handled, transferred, or stored a piece of evidence, establishing that it has not been altered or contaminated.
Cross-contaminationThe unintended transfer of material from one evidence sample or location to another, which can invalidate forensic analysis and destroy the evidentiary value of a sample.
StriationA fine, parallel scratch or furrow left in a toolmark by microscopic irregularities on the tool's surface; striations can be unique to an individual tool and used for identification.

Worked examples3

1. You have a package of dental stone that specifies a mix ratio of 300 g of powder to 100 mL of water. You estimate the shoe impression will require approximately 1,500 mL of mixed material to fill and reinforce. How many grams of powder and how many milliliters of water do you need?
  1. Step 1 — Understand the ratio: 300 g powder + 100 mL water produces one unit of mix. We can treat this as: for every 100 mL of water used, you need 300 g of powder.
  2. Step 2 — Determine the scale factor: The target volume is 1,500 mL of mixed material. The base batch (300 g powder + 100 mL water) yields approximately 400 mL of mix (powder displaces volume when it dissolves). Scale factor = 1,500 ÷ 400 = 3.75.
  3. Step 3 — Calculate water: 100 mL × 3.75 = 375 mL of water.
  4. Step 4 — Calculate powder: 300 g × 3.75 = 1,125 g of powder.
  5. Step 5 — Verify: 1,125 g powder + 375 mL water → approximately 1,500 mL of mix. This matches the target.
Answer: You need approximately 1,125 g of dental stone powder and 375 mL of water to produce roughly 1,500 mL of mixed casting material at the specified ratio.
2. You are photographing a toolmark on a metal coupon. The mark is 18 mm long. Your camera sensor in macro mode captures a field of view 36 mm wide. What scale ratio (magnification) are you working at if you fill the frame width with the 36 mm field?
  1. Step 1 — Identify what fills the frame: the sensor captures 36 mm of real-world width.
  2. Step 2 — A full-frame 35 mm sensor is 36 mm wide. So the image width on the sensor = 36 mm; the real-world width captured = 36 mm.
  3. Step 3 — Scale ratio = image size ÷ object size = 36 mm ÷ 36 mm = 1:1.
  4. Step 4 — The 18 mm mark will appear as 18 mm on the sensor image, confirming life-size (1:1) reproduction.
  5. Step 5 — Record '1:1 macro' in your photographic notes so the examiner knows the images are true to scale.
Answer: The scale ratio is 1:1 (life-size). The 18 mm toolmark is reproduced at 18 mm on the sensor, and this ratio must be recorded in case notes and reflected in the photo scale placed in the frame.
3. Dental stone requires a minimum of 30 minutes of undisturbed curing at 70 °F. The ambient temperature at your outdoor scene is 45 °F. General guidance states that for every 10 °F drop below 70 °F, add approximately 15 minutes to the curing time. How long should you wait before excavating the cast?
  1. Step 1 — Calculate the temperature difference: 70 °F − 45 °F = 25 °F below the baseline.
  2. Step 2 — Determine the number of 10 °F increments: 25 °F ÷ 10 °F = 2.5 increments.
  3. Step 3 — Calculate additional curing time: 2.5 × 15 minutes = 37.5 minutes of additional time.
  4. Step 4 — Add to baseline: 30 minutes + 37.5 minutes = 67.5 minutes.
  5. Step 5 — Round up for safety: wait at least 68 minutes (just over 1 hour) before excavating. When in doubt, wait longer — a broken cast cannot be repaired.
Answer: At 45 °F you should wait approximately 68 minutes (rounding up from 67.5) before excavating the cast, to account for the slowed chemical reaction at lower temperatures.

The math in this lesson1

Learners scale a 300 g powder to 100 mL water casting ratio up to a 1,500 mL batch.
6.RP.3
In class: Use an electrostatic lifting device (ESDA) on a dusty surface to recover a latent shoe impression; photograph the lift before and after collection, complete documentation, and store the lift in a rigid container to prevent distortion. · Mix dental stone to package-specified consistency, pour a cast of an instructor-prepared shoe impression in sand or soil, allow to cure, excavate, clean, label, and package for transport without breaking the cast. · Examine a set of toolmark exemplars on soft metal coupons (created by instructor with screwdrivers and pliers); photograph with scale, cut out the coupon section containing the mark without touching the mark surface, wrap in protective foam, and complete the evidence form.
Materials: Electrostatic lifting device (ESDA); dental stone powder; mixing bowls; water; shoe-impression forms in sand trays; rubber spatulas; toolmark coupons (soft aluminum); camera with macro lens; photo scales; rigid evidence containers; foam padding; evidence forms.
Check: Each student submits one ESDA lift, one dental stone cast, and one packaged toolmark coupon with completed forms; instructor checks against a 15-point rubric covering documentation accuracy, packaging integrity, and absence of damage to evidence; pass = 12 of 15 points.

Practice check7

Answer each question, then check it. Nothing is recorded; this is your own practice.

Crime Scene Documentation and Evidence Collection
1. When using an electrostatic lifting device (ESDA) to recover a dust impression, what is the correct immediate action after the lift film is removed from the surface?
Impression Evidence
2. A technician is about to mix dental stone for casting a shoe impression in moist soil. Which mixing sequence is correct?
Evidence Collection and Preservation
3. Why should trace evidence such as hair and fiber samples be stored in paper envelopes rather than sealed plastic bags?
Crime Scene Photography and Documentation
4. An investigator is photographing a tire-track impression for documentation purposes. Which lighting technique best reveals the depth and fine detail of the impression?
Chain of Custody and Documentation
5. A technician completes evidence collection but makes a written error on the chain-of-custody form. What is the correct procedure for correcting the error?
Impression Evidence
6. During dental stone casting at a cold outdoor scene (45 °F), a technician excavates the cast only 30 minutes after pouring. What is the most likely consequence?
Safety and Personal Protective Equipment
7. Which personal protective equipment (PPE) is required when mixing dental stone powder in an outdoor setting?
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