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AGR-HORT49-05 · Lesson 5 · 14 clock hours · practice

Plant Propagation: Seed, Cuttings, and Division

Objective. Students propagate plants by three methods—direct seeding, stem-tip cuttings, and crown division—following sanitary protocol, and achieve a germination or rooting result documented in a propagation log.

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Plant Propagation: Seed, Cuttings, and Division

Propagation is how growers multiply plants reliably and economically. In this practice lesson you will use three fundamental methods—direct seeding, stem-tip cuttings, and crown division—and document every result in a propagation log. Accurate records are not optional; commercial greenhouse production depends on knowing what worked, when, and why.

Sanitation: The Foundation of Every Propagation Method

Disease organisms spread faster in propagation areas than anywhere else in the greenhouse because the environment—warm, humid, low-light—favors pathogens and the plant material has no protective bark or cuticle. Before touching any plant material, sanitize all cutting blades and bench surfaces with 70% isopropyl alcohol. Let the blade air-dry before use; alcohol residue can damage tissue. Replace solution when it becomes cloudy. Never use the same unwashed blade on a second stock plant without re-sanitizing. Wear nitrile gloves when handling rooting hormone to avoid skin exposure.

Seed Propagation

Seeds are the most economical starting point for many annuals and vegetables. The key variables are medium, moisture, temperature, and depth.

Propagation mix is a soilless blend—typically peat or coir, perlite, and vermiculite—that drains freely while holding enough moisture for germination. Never use field soil; it compacts and carries pathogens.

Filling 72-cell plug trays: Overfill the tray, then strike off the excess with a straight edge. Firm gently—cells should be full but not compacted. Pre-moisten the medium until it holds its shape when squeezed but releases only a drop or two of water.

Sowing: Read the seed packet. Depth is usually one to two times the seed diameter. Fine seeds (petunia, begonia) are sown on the surface and not covered. Place one or two seeds per cell depending on germination rate expectations. Label every tray immediately with crop name, variety, and sow date—memory is not a propagation tool.

Germination environment: Most warm-season annuals germinate best at 70–75 °F substrate temperature. A germination chamber or misted bench maintains humidity. Check daily. Remove trays from the germination chamber promptly once 50–60% of seeds have emerged to prevent stretching.

Recording results: Count emerged seedlings at Day 7 and Day 14. Germination percentage = (number of emerged seedlings ÷ number of seeds sown) × 100. Record both counts in your propagation log.

Stem-Tip Cutting Propagation

Stem-tip (terminal) cuttings reproduce a plant genetically identical to the parent—critical when maintaining a specific flower color or growth habit.

Selecting cuttings: Choose healthy, pest-free stock plants. A good cutting is 3–4 inches long, has at least two nodes, and is neither too soft (wilts easily) nor too woody (roots slowly). Take cuttings in the morning when plants are fully turgid.

Making the cut: Use a clean, sharp blade. Cut just below a node at a 45-degree angle to maximize the rooting surface and improve drainage away from the cut end. Remove leaves from the bottom one-third of the cutting to prevent rot where the stem enters the medium.

Rooting hormone: IBA (indole-3-butyric acid) is the industry standard active ingredient. Powder and gel formulations are both widely used. Dip the basal cut end into powder (tap off excess) or apply gel with a clean applicator. Do not double-dip the container—pour a small amount into a separate dish to prevent contaminating the stock supply. Rooting hormone concentration varies by species; follow label directions.

Sticking and environment: Insert cuttings into pre-moistened propagation medium in 50-cell trays at a depth that keeps them upright—typically 1–1.5 inches. Tent with clear poly film to maintain humidity above 85%. Avoid direct sun under the tent; diffuse light prevents overheating. Mist the interior if condensation disappears. Evaluate rooting at 14–21 days by gently tugging a cutting; resistance indicates root formation.

Crown Division

Crown division is the simplest vegetative method for clump-forming herbaceous perennials such as hostas and daylilies. It requires no rooting hormone and no special environment, but each division must be viable.

What makes a division viable? Each piece must have an intact root system and at least one growth point (bud, eye, or shoot). A division with roots but no growth point will not grow. A shoot with no roots will die.

Technique: Lift or remove the plant from its container. Use a clean, sharp spade, knife, or hand pruners to cut through the crown. Minimize tearing. Aim for four or more divisions of roughly equal size. Trim any dead or damaged roots. Pot immediately into 4-inch pots with moist growing medium. Water in gently. Label each pot with species name and division date.

Assessment: New shoot emergence within two weeks indicates a successful, viable division. Record emergence date and status in your propagation log.

Completing Your Propagation Log

Your log must include: date of activity, species and variety, propagation method, number of units started, count and percentage at each evaluation date, and any observations (disease, environmental deviation, technique notes). A complete log is your evidence of competency and your reference for future crops.

Key terms9

Propagation mixA soilless growing medium—typically peat or coir, perlite, and vermiculite—designed for seed germination and rooting cuttings; it drains freely while retaining adequate moisture and is free of field pathogens.
IBA (indole-3-butyric acid)The active ingredient in most commercial rooting hormone products; a plant auxin that stimulates adventitious root formation at the base of a stem cutting.
NodeThe point on a stem where leaves, buds, or branches attach; stem-tip cuttings are cut just below a node because root initiation begins most readily at this tissue.
Germination percentageThe proportion of seeds sown that successfully emerge, calculated as (emerged seedlings ÷ seeds sown) × 100; a standard measure of propagation success.
Crown divisionA vegetative propagation method in which a clump-forming herbaceous perennial is physically separated into sections, each with roots and at least one growth point, to produce new independent plants.
Adventitious rootsRoots that develop from non-root tissue—such as the stem of a cutting—in response to wounding and rooting hormone; the mechanism by which stem cuttings become self-sufficient plants.
Tenting (poly tent)The practice of covering trays of cuttings with clear polyethylene film to trap humidity and reduce water loss through the leaves before roots have formed.
Plug trayA multi-cell tray (commonly 72-cell or 128-cell) used for starting seeds or rooting cuttings; each cell produces one transplant-ready unit with a discrete root ball.
Viable divisionA section of a divided perennial plant that possesses both an intact root system and at least one growth point (bud, eye, or emerging shoot), giving it the capacity to grow as an independent plant.

Worked examples4

1. A student sows 72 cells in a plug tray, one seed per cell. At Day 14, she counts 54 emerged seedlings. What is the germination percentage, and does it meet the 70% minimum?
  1. Identify values: seeds sown = 72, seedlings emerged = 54.
  2. Apply the formula: germination % = (emerged ÷ sown) × 100.
  3. Calculate: (54 ÷ 72) × 100 = 75%.
  4. Compare to standard: 75% is greater than the 70% minimum.
Answer: Germination percentage is 75%. The tray meets the 70% minimum standard.
2. A student sticks 10 stem-tip cuttings of coleus. At Day 21, she evaluates each cutting with a gentle tug test. Eight cuttings show resistance indicating rooting; two pull out freely. What is the rooting percentage, and does it meet the 70% minimum?
  1. Identify values: cuttings stuck = 10, cuttings rooted = 8.
  2. Apply the formula: rooting % = (rooted ÷ stuck) × 100.
  3. Calculate: (8 ÷ 10) × 100 = 80%.
  4. Compare to standard: 80% is greater than the 70% minimum.
Answer: Rooting percentage is 80%. The batch meets the 70% minimum standard.
3. A student divides a hosta crown into 6 sections and pots each one. At Day 14, new shoots have emerged on 5 of the 6 pots. What percentage of divisions is viable, and does every division need to show emergence for the activity to be considered successful?
  1. Identify values: divisions made = 6, showing new shoot emergence = 5.
  2. Calculate viability percentage: (5 ÷ 6) × 100 ≈ 83%.
  3. Review the success criterion: the lesson standard requires all four minimum divisions to show new shoot emergence; the student made 6 divisions and all 4 minimum viable divisions (any 4 of 6) must emerge.
  4. Since 5 of 6 show emergence (exceeding the minimum of 4), the crown division activity meets the completion standard.
Answer: Approximately 83% of divisions are viable. Because at least four divisions show new shoot emergence, the activity meets the required standard.
4. A student is filling 72-cell trays for two species. Species A requires one seed per cell; Species B has a low expected germination rate so the student sows two seeds per cell in 36 cells. How many seeds total does the student need for one complete tray of each species?
  1. Species A: 72 cells × 1 seed per cell = 72 seeds.
  2. Species B: 36 cells × 2 seeds per cell = 72 seeds.
  3. Total seeds needed: 72 + 72 = 144 seeds.
Answer: The student needs 144 seeds total: 72 for Species A and 72 for Species B.

The math in this lesson1

Learners compute germination and rooting percentages, such as 54 of 72 seeds, against a 70% standard.
7.RP.3
Brush up in the math halls at newSchoolWorks.com:
In class: Seed propagation: students fill 72-cell plug trays with propagation mix, sow seeds of two species at the correct depth and spacing (per seed packet instructions), label trays with crop name and sow date, and place trays in a germination chamber or misted bench; germination percentage is recorded at 7 and 14 days. · Stem-tip cutting propagation: students take 10 cuttings from stock plants (e.g., impatiens, coleus, or geranium), make a clean basal cut below a node with a sanitized blade, dip in rooting hormone (appropriate concentration for the species), stick in moistened propagation medium in cell trays, and tent with clear poly; rooting percentage is evaluated at 14–21 days. · Crown division: students divide one herbaceous perennial (e.g., hosta or daylily) into a minimum of four viable divisions, ensuring each has roots and at least one growth point, then pot and label each division; viability is assessed at 2 weeks by new shoot emergence.
Materials: 72-cell plug trays, 50-cell propagation trays, propagation mix, seeds (two species), stock plants for cuttings, rooting hormone powder or gel (IBA-based), sanitized cutting blades, isopropyl alcohol (70%), herbaceous perennial divisions, 4-inch pots, clear poly film, misting nozzle, propagation log sheets, plant labels, permanent markers
Check: Student achieves minimum 70% germination for seeded trays by Day 14, minimum 70% rooting on cuttings by Day 21, and all four divisions show new shoot emergence by Day 14; propagation log is complete with dates, species, method, and percentage outcomes.

Practice check8

Answer each question, then check it. Nothing is recorded; this is your own practice.

Greenhouse Production and Sanitation Practices
1. Why must cutting blades be sanitized with 70% isopropyl alcohol between stock plants during cutting propagation?
Plant Propagation Techniques
2. A stem-tip cutting is taken just below a node because:
Greenhouse Production Calculations
3. A student sows 72 seeds (one per cell) and records 45 emerged seedlings at Day 14. What is the germination percentage?
Pesticide and Chemical Handling (Propagation Inputs)
4. When applying rooting hormone powder to stem-tip cuttings, the best practice is to:
Plant Propagation Techniques
5. Which characteristic is essential for a crown division to be considered viable?
Greenhouse Environment Management
6. A clear poly tent is placed over a tray of freshly stuck cuttings primarily to:
Greenhouse Production and Environment Management
7. Which propagation medium characteristic is most important for germinating seeds and rooting cuttings in a commercial greenhouse setting?
Greenhouse Production and Environment Management
8. According to industry best practices, at what point should plug trays be removed from a germination chamber after sowing seeds?
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