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newSchoolWorks.com › VoTechWorks › Agriculture, Environment & Public Service › Aquaculture & Marine Science Tech › Hatchery Operations: Broodstock to Juvenile Production › Lesson 2
AGR-AQUA50-04 · Lesson 2 · 20 clock hours · lab

Water Delivery and Recirculating Aquaculture Systems (RAS)

Objective. Students will operate pumps, valves, and biofilter units in a functioning RAS, adjust flow rates to target specifications, and perform routine mechanical checks including impeller inspection and airline clearing.

⚠ Pending professional review

This lesson, its worked examples and its practice questions has not yet been verified by a licensed professional in the field or by a certification or approval body. It is a study draft and may contain errors. Do not use it as instructions for work on a job site or on live equipment. Always follow your instructor, your supervising professional, and current official references and regulations.

VoTechWorks program approval and accreditation are still in progress. Credentials are issued only by the certifying bodies named in each program, after their own exams.

Water Delivery and Recirculating Aquaculture Systems (RAS)

A recirculating aquaculture system keeps water moving through a closed loop rather than discarding it after one pass. Water flows from fish or larval tanks, through mechanical and biological filtration, through aeration or oxygenation, and back to the tanks. Understanding every component of that loop — and being able to respond when something goes wrong — is a core hatchery skill.

How Water Moves Through a RAS

Pumps are the heart of any RAS. A centrifugal pump draws water in through the impeller eye and accelerates it outward to create pressure that pushes flow through the system. The relationship between flow rate and pressure is described by a pump curve chart: as back-pressure rises (for example, when a filter becomes loaded), flow rate drops. Always consult the pump curve and the manufacturer manual when troubleshooting low flow.

Water is distributed to tanks through header pipes and standpipes. Gate valves on each standpipe let you throttle flow to individual tanks. Turning a gate valve clockwise reduces flow; counterclockwise opens it. Always move valves slowly — sudden changes create water hammer and can stress fish.

Measuring and Adjusting Flow Rate

The simplest field method is the bucket-and-stopwatch test: hold a graduated bucket under the standpipe outlet, time how many seconds it takes to collect a known volume, then calculate flow rate. For example, if 5 gallons collects in 20 seconds, the flow rate is 5 ÷ 20 = 0.25 gal/s, or 15 gal/min. Compare your measured value to the posted target flow for that tank and adjust the gate valve as needed. Re-measure after every adjustment and record both the before and after readings in the lab log. Your target is to be within 10% of the posted value.

Biofilters: Function and Backwashing

Biological filtration converts toxic ammonia (excreted by fish) to nitrite, then to the less toxic nitrate, through colonies of nitrifying bacteria that grow on filter media. Two common media types in hatcheries are bead filters (floating plastic beads) and drum filters (rotating fine-mesh screens that handle solids mechanically).

Over time, solids accumulate and reduce flow through the biofilter. A backwash cycle flushes accumulated solids out while preserving as much bacterial biomass as possible. Always follow the written SOP exactly:

1. Notify the hatchery manager before isolating any filter.

2. Close the inlet valve to isolate the filter from the main flow.

3. Open the backwash port and run the backwash per the manufacturer cycle time — do not guess.

4. Close the backwash port and slowly reopen the inlet valve.

5. Collect a water sample immediately before and after backwash and measure turbidity. A lower turbidity reading after backwash confirms the solids load was reduced.

6. Record all readings, times, and any observations in the lab log.

Never skip isolation steps. Backwashing a filter while it is still in full service can send a turbidity pulse directly to larval tanks, causing gill damage or mortality.

Pump Failure Response

Header pump failure is the most time-critical emergency in a RAS. Fish can survive minutes to hours without filtration depending on density and temperature, but they need water movement and dissolved oxygen continuously. When a pump alarm sounds or flow stops:

1. Confirm the failure: check the alarm panel for which pump has tripped.

2. Switch to the backup pump: locate the backup pump isolation valve or switch; open the backup outlet valve, then start the backup pump motor.

3. Verify flow is restored: observe standpipe flow and check the flow meter if installed.

4. Notify the hatchery manager immediately — even if flow is restored. The underlying fault must be diagnosed.

5. Document the event: record time of alarm, time of switchover, time of notification, and any observations about the failed pump.

The entire response must be completed within five minutes. Practice the procedure until it is automatic.

Impeller Inspection and Airline Clearing

During scheduled pump maintenance, always follow lockout/tagout (LOTO) procedures before opening any pump housing. Isolate electrical power at the breaker, apply a lockout device, and verify that the pump cannot restart before reaching inside. Impeller inspection checks for wear, debris, or cavitation damage — a pitted or cracked impeller reduces efficiency and can fail suddenly.

Airlines (diffuser lines, air-stone tubing) clog with biofilm over time. Clear them by briefly back-purging with clean air or by soaking in a dilute acid or enzyme solution per the facility protocol. Never blow air lines with your mouth.

Safety Points

Key terms10

Recirculating Aquaculture System (RAS)A closed-loop water system that treats and reuses hatchery water through mechanical filtration, biofiltration, aeration, and temperature control rather than continuous flow-through discharge.
Pump curveA manufacturer's chart showing the relationship between a pump's flow rate and the head (back-pressure) it works against; used to predict or troubleshoot pump performance.
Gate valveA sliding-gate valve used to throttle or stop water flow in a pipeline; adjusted slowly to avoid water hammer and stress to fish.
BiofilterA filtration unit colonized by nitrifying bacteria that convert ammonia to nitrite and then to nitrate, making water safe for recirculation.
BackwashA controlled procedure that reverses or redirects flow through a filter to flush accumulated solids while preserving the bacterial colony on the media.
TurbidityA measure of water cloudiness caused by suspended particles; used here as an indicator of solids load before and after a biofilter backwash cycle.
ImpellerThe rotating component inside a centrifugal pump that transfers energy to the water; wear or damage to the impeller reduces flow and efficiency.
Lockout/Tagout (LOTO)An OSHA-required procedure in which a worker isolates an energy source (electrical, hydraulic) with a physical lock and tag before performing maintenance, preventing accidental re-energization.
StandpipeA vertical pipe inside or at the tank outlet that controls water depth in the tank; flow to individual standpipes is adjusted with a gate valve on the supply side.
NitrificationThe two-step biological process in which ammonia-oxidizing bacteria convert ammonia (NH₃) to nitrite (NO₂⁻), then nitrite-oxidizing bacteria convert nitrite to nitrate (NO₃⁻).

Worked examples4

1. During the flow-rate calibration exercise you time how long it takes to fill a 5-gallon bucket at Standpipe 3. You collect 5 gallons in 25 seconds. The posted target flow for that standpipe is 14 gal/min. Is the current flow within 10% of target, and which direction should you adjust the gate valve?
  1. Step 1 – Convert the measured rate to gal/min: flow = (5 gal ÷ 25 s) × 60 s/min = 12.0 gal/min.
  2. Step 2 – Calculate the acceptable range (±10% of 14 gal/min): lower limit = 14 × 0.90 = 12.6 gal/min; upper limit = 14 × 1.10 = 15.4 gal/min.
  3. Step 3 – Compare: 12.0 gal/min is below the lower limit of 12.6 gal/min, so the flow is outside the acceptable range.
  4. Step 4 – Determine valve direction: flow is too low, so you must open the gate valve slightly (turn counterclockwise) to increase flow, then re-measure.
Answer: Measured flow is 12.0 gal/min, which is below the acceptable range of 12.6–15.4 gal/min. Open (counterclockwise) the gate valve slightly and re-measure.
2. After adjusting Standpipe 3, you collect 5 gallons in 21 seconds. Confirm whether the flow now falls within 10% of the 14 gal/min target.
  1. Step 1 – Convert: flow = (5 ÷ 21) × 60 = 300 ÷ 21 ≈ 14.3 gal/min.
  2. Step 2 – Acceptable range is 12.6–15.4 gal/min (from the previous example).
  3. Step 3 – Compare: 14.3 gal/min falls between 12.6 and 15.4 gal/min.
  4. Step 4 – Conclusion: the flow is within 10% of target. Record the before value (12.0 gal/min) and after value (14.3 gal/min) in the lab log.
Answer: 14.3 gal/min is within the 12.6–15.4 gal/min acceptable range. The adjustment is complete; record both readings.
3. You collect a turbidity reading of 18 NTU before backwash and 6 NTU after backwash. What is the percentage reduction in turbidity, and what does this indicate?
  1. Step 1 – Calculate the reduction: 18 NTU − 6 NTU = 12 NTU removed.
  2. Step 2 – Calculate percentage reduction: (12 ÷ 18) × 100 = 66.7%.
  3. Step 3 – Interpret: a roughly 67% drop in turbidity indicates that the backwash successfully flushed a significant solids load from the biofilter.
  4. Step 4 – Record both values and the percentage in the lab log as required by the SOP.
Answer: Turbidity was reduced by approximately 66.7%, from 18 NTU to 6 NTU, confirming an effective backwash cycle.
4. Your posted target for Standpipe 6 is 10 gal/min. You measure 5 gallons collected in 27 seconds. Calculate the flow rate and determine the percentage deviation from target.
  1. Step 1 – Convert: flow = (5 ÷ 27) × 60 ≈ 11.1 gal/min.
  2. Step 2 – Calculate deviation from target: difference = 11.1 − 10.0 = 1.1 gal/min above target.
  3. Step 3 – Calculate percentage deviation: (1.1 ÷ 10.0) × 100 = 11.1%.
  4. Step 4 – Compare to criterion: 11.1% exceeds the 10% tolerance, so the flow must be slightly reduced by closing the gate valve (clockwise) and re-measured.
Answer: Flow is approximately 11.1 gal/min, which is 11.1% above target — just outside the ±10% limit. Close the gate valve slightly and re-measure.

The math in this lesson2

Learners convert a 5 gal per 25 s bucket test to gal/min.
6.RP.37.RP.3
Brush up in the math halls at newSchoolWorks.com:
In class: Flow-rate calibration exercise: students use a graduated bucket and stopwatch to measure actual flow at six standpipes in the larval room, then adjust gate valves to bring each within 10% of the posted target flow, recording before-and-after measurements. · Biofilter backwash: students follow the written SOP to isolate a bead or drum biofilter, perform a backwash cycle, collect a pre- and post-backwash water sample for turbidity comparison, and record findings in the lab log. · Simulated pump failure response: instructor cuts power to a header pump; students execute the emergency response SOP—switching to backup pump, checking alarm panel, notifying the hatchery manager, and documenting the event—within a five-minute window.
Materials: RAS training unit or operational hatchery RAS, graduated 5-gallon buckets, stopwatches, turbidity meter, gate valves, pump curve charts, Pentair or equivalent pump manuals, emergency response SOP cards, lab log notebooks
Check: All six standpipe flows adjusted to within 10% of target; backwash SOP completed in correct sequence with zero skipped steps; emergency response completed within five minutes — all three criteria must be met to pass.

Practice check8

Answer each question, then check it. Nothing is recorded; this is your own practice.

Hatchery Operations and Water System Management
1. During the bucket-and-stopwatch test at a standpipe, a student collects 5 gallons in 30 seconds. What is the flow rate in gallons per minute?
Hatchery Operations and Water System Management
2. The posted target flow for a larval tank standpipe is 20 gal/min. Which of the following measured flow rates falls within the required ±10% tolerance?
Hatchery Operations and Water System Management
3. Before backwashing a bead biofilter, a technician must first:
Mechanical Systems and Equipment
4. A pump curve chart is most useful in a RAS hatchery for:
Emergency Response and Safety Procedures
5. During a simulated pump failure, the first action a hatchery technician should take after confirming which pump has tripped is:
Occupational Safety and Hazard Control
6. Which safety procedure is mandatory before opening a pump housing to inspect the impeller?
Hatchery Operations and Water System Management
7. A technician notices that turbidity is higher than normal in the larval tank water after a biofilter backwash. The most likely cause is:
Hatchery Operations and Water System Management
8. What does a reading of high turbidity in a pre-backwash sample compared to a low turbidity in the post-backwash sample indicate about the biofilter?
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