🚧 Under construction — VoTechWorks is being built. A free tech program — complimentary, as our commitment to get America working.
voTechWorksGrade 13 · a vocational technology learning center, by urbanicity
newSchoolWorks.com › VoTechWorks › Health & Personal Services › Sterile Processing Technician › Infection Control for Sterile Processing › Lesson 3
HLT-STER66-06 · Lesson 3 · 14 clock hours · demo

Standard Precautions and Transmission-Based Precautions in Practice

Objective. Students will demonstrate the correct selection and donning/doffing sequence for PPE required under Standard Precautions and each Transmission-Based Precaution category when receiving instruments from isolation patients.

⚠ Pending professional review

This lesson, its worked examples and its practice questions has not yet been verified by a licensed clinician in the field or by a certification or approval body. It is a study draft and may contain errors. Do not use it to make decisions about any patient or medication. Always follow your instructor, your supervising professional, and current official references and regulations.

VoTechWorks program approval and accreditation are still in progress. Credentials are issued only by the certifying bodies named in each program, after their own exams.

Why Precautions Matter in Sterile Processing

Sterile Processing Technicians (SPTs) handle instruments that have been used on patients—including patients under isolation precautions. Understanding and correctly applying Standard Precautions and Transmission-Based Precautions protects you from exposure to infectious agents and prevents cross-contamination in the decontamination area. Patient safety and worker safety are inseparable: a lapse in PPE technique can spread a pathogen from a soiled instrument tray to you, to coworkers, or—through improperly handled equipment—back to other patients.

Standard Precautions

Standard Precautions apply to every patient encounter and every soiled instrument, regardless of known diagnosis. They are based on the principle that blood, all body fluids, non-intact skin, and mucous membranes are potentially infectious.

For sterile processing, Standard Precautions require:

These elements are non-negotiable for every tray that enters the decontamination zone.

Transmission-Based Precautions: An Overview

When a patient's known or suspected illness requires additional controls beyond Standard Precautions, the care team assigns Transmission-Based Precautions. The CDC recognizes three categories: Contact, Droplet, and Airborne. As an SPT receiving instruments from these patients, you must understand which additional PPE each category requires.

Contact Precautions

Used for pathogens spread by direct or indirect touch—including organisms such as MRSA (methicillin-resistant Staphylococcus aureus), VRE (vancomycin-resistant Enterococcus), Clostridioides difficile, and certain multidrug-resistant organisms (MDROs).

PPE additions beyond Standard Precautions: Gown and gloves must be donned before entering the workspace or contacting the tray. Instruments from Contact Precaution patients should be transported in clearly labeled, sealed/closed containers. Exercise extreme care not to touch environmental surfaces with contaminated gloves.

For C. difficile specifically: alcohol-based hand rubs are not effective against C. diff spores. Handwashing with soap and water is required after removing gloves.

Droplet Precautions

Used for pathogens transmitted by large respiratory droplets (generally traveling about 3–6 feet), such as influenza, pertussis, and certain streptococcal infections.

PPE additions: A surgical mask (in addition to standard eye protection or face shield) must be worn when handling trays and instruments that may carry respiratory secretions. Instruments from the airway or upper respiratory tract of these patients carry elevated splash risk during decontamination.

Airborne Precautions

Used for pathogens spread by small airborne particles that can remain suspended for longer distances—tuberculosis (Mycobacterium tuberculosis), measles, and varicella (chickenpox).

PPE additions: An N95 respirator (or higher-level respirator) replaces the surgical mask. N95 respirators must be fit-tested before use, and you should perform a user seal check each time you don one. Never substitute a surgical mask when Airborne Precautions apply.

Donning Sequence (General)

Don PPE in this order to minimize self-contamination:

1. Gown – Tie at neck, then waist.

2. Mask or N95 respirator – Secure straps; perform seal check for N95.

3. Eye protection / face shield – Position over mask.

4. Gloves – Pull cuffs over gown cuffs.

Doffing Sequence (General)

Doffing carries the highest contamination risk because the outside of PPE is considered contaminated. Proceed in this order:

1. Gloves – Peel the first glove inside-out; use the bare-hand-to-inner-surface technique for the second.

2. Hand hygiene – Perform immediately.

3. Face shield or goggles – Remove by the clean headband or earpieces; avoid touching the front.

4. Gown – Untie, roll outward away from you, and discard without shaking.

5. Mask or respirator – Remove by the straps/ties only; do not touch the front.

6. Hand hygiene – Perform again.

Critical safety rule: At no point during doffing should your hands (gloved or bare) touch your face, neck, hair, or any clean surface in the work environment. Any such contact is a critical contamination error.

Handling Instrument Trays from Isolation Patients

When a tray is labeled with an isolation category:

Hand Hygiene: The Foundation

Hand hygiene is performed before donning and after every step of doffing. Use alcohol-based hand rub for most organisms; use soap and water for C. difficile and when hands are visibly soiled. This single step prevents the most common route of pathogen spread in healthcare settings.

Competency Expectations

For your live competency check, you must complete the full donning and doffing sequence with zero critical contamination errors. Practice the sequence until it is automatic—muscle memory protects both you and your patients.

Key terms10

Standard PrecautionsA baseline set of infection control practices applied to every patient and every soiled item, treating blood and all body fluids as potentially infectious regardless of diagnosis.
Transmission-Based PrecautionsAdditional infection control measures layered on top of Standard Precautions when a patient's pathogen requires extra controls; categories are Contact, Droplet, and Airborne.
Contact PrecautionsPrecautions used for pathogens spread by direct or indirect contact (e.g., MRSA, VRE, C. difficile); require gown and gloves before touching the patient's instruments or environment.
Droplet PrecautionsPrecautions used for pathogens spread by large respiratory droplets; require a surgical mask in addition to standard eye protection.
Airborne PrecautionsPrecautions used for pathogens spread via small airborne particles (e.g., tuberculosis, measles); require a fit-tested N95 or higher-level respirator.
N95 RespiratorA tight-fitting particulate respirator that filters at least 95% of airborne particles; requires fit testing and a user seal check each use; required under Airborne Precautions.
DoffingThe process of removing PPE in a specific sequence designed to prevent transferring contaminants from the outer (dirty) surface of PPE to skin, clothing, or clean surfaces.
Multidrug-Resistant Organism (MDRO)A microorganism—such as MRSA or VRE—that has developed resistance to multiple antimicrobial agents and often requires Contact Precautions to prevent spread.
Critical Contamination ErrorDuring doffing, any unintended contact of contaminated PPE or hands with the face, neck, hair, or a clean surface; a serious safety breach in the competency evaluation.
Seal CheckA quick user-performed test done each time an N95 respirator is donned to verify that air moves through the filter rather than leaking around the edges of the mask.
In class: Instructor demonstrates full donning and doffing sequence for Contact, Droplet, and Airborne Precaution PPE sets; students observe and annotate a sequencing worksheet, noting contamination risks at each step. · Students practice donning and doffing three times each (rotating PPE sets) while a partner uses a contamination-check checklist to flag any self-contamination events such as glove touch to face or improper gown removal order. · Scenario role-play: instructor calls out an isolation category (e.g., 'incoming tray from a VRE Contact Precaution patient'); student must verbalize PPE selection rationale, don correctly, and describe handling differences for the tray before doffing; debrief follows.
Materials: Gloves (multiple sizes), surgical masks, N95 respirators, face shields, isolation gowns, shoe covers, PPE donning/doffing sequencing poster, contamination-check checklists, CDC Isolation Precautions guideline summary handout
Check: Live competency check using a standardized donning/doffing checklist; student must complete the correct sequence with zero critical contamination errors (defined as any touch to face, neck, or clean surface during doffing); one repeat attempt allowed, then remediation.

Practice check8

Answer each question, then check it. Nothing is recorded; this is your own practice.

Infection Control
1. A labeled tray arrives from a patient on Contact Precautions for VRE. Before handling the tray, which PPE must you don in addition to your standard decontamination PPE?
Infection Control
2. During doffing, which step must occur IMMEDIATELY after removing your gloves?
Infection Control
3. A tray is received from a patient with confirmed pulmonary tuberculosis. Which respiratory protection is required when handling this tray?
Infection Control
4. After decontaminating instruments from a patient with Clostridioides difficile infection, you remove your gloves and are ready for hand hygiene. Which method is MOST appropriate?
Infection Control
5. Which of the following actions during doffing would be classified as a critical contamination error?
Infection Control
6. A student is unsure what precaution category applies to an incoming tray. The transport container label reads 'Droplet Precautions.' Which additional PPE item does the student need beyond standard decontamination eye protection and gown?
Infection Control
7. In the correct general donning sequence, when should gloves be put on relative to the gown?
Infection Control
8. Standard Precautions in the sterile processing decontamination area apply to:
Record this lesson on my transcript. Sign in with your key and enrollment email to record lessons (enroll free if you have no key). This page stays open to read and practise.